Determining optimal range of reduction rates for nitrogen fertilization based on responses of vegetable yield and nitrogen losses to reduced nitrogen fertilizer application.

Vegetable production is commonly accompanied by high nitrogen fertilizer rates but low nitrogen use efficiency in China. Reduced fertilization has been frequently recommended in existing studies as an efficient measurement to avoid large amount of nutrient loss and subsequent nonpoint source pollution. However, the reported responses of vegetable yield and nitrogen losses to reduced fertilization rates varied in a large range, which has resulted into large uncertainties in the potential benefits of those recommended reduction rates. Thus, we constructed the relationship between responses of nitrogen losses and vegetable yield to reduced nitrogen fertilization rates to determine the optimal range of reduction rates for nitrogen fertilization in a proportional form based on data reported in literatures across China's mainland, and evaluated the roles of greenhouse, managing options, and vegetable species on the responses. The relationships were constructed separately for 4 subregions: Northern arid and semiarid, loess plateau regions (NSL), Temperate monsoon zone (TMZ), Southeast monsoon zone (SMZ), Southwest zone (SWZ). The optimal nitrogen fertilizer reduction range for the TMZ, SMZ and SWZ were 51 % to 67 %, 40 % to 66 % and 54 % to 80 %, respectively and no reduction for NSL. Vegetable yields were not be sacrificed when fertilizations were reduced within the optimal ranges. Greenhouse and managing options showed no significant effect on the responses of both vegetable yield and nitrogen losses by the optimal reduction range but vegetable species played a relatively important role on the responses of vegetable yield. This indicated that the optimal reduction rates can be effective on reducing nitrogen loss in both open-field and greenhouse conditions across China's mainland without extra managing options. Therefore, the optimal reduction rates can still serve as a good starting point for making regional plans of nitrogen reduction that help balancing the chasing of high vegetable yield and low nitrogen loss.

Urbanization and land use regulate soil vulnerability to antibiotic contamination in urban green spaces.

The presence of antibiotics in environment is an emerging concern because of their ubiquitous occurrence, adverse eco-toxicological effects, and promotion of widespread antibiotic resistance. Urban soil, which plays a noticeable role in human health, may be a reservoir of antibiotics because of intensive human disturbance. However, little is understood about the vulnerability of soil to antibiotic contamination in urban areas and the spatial-temporal characteristics of anthropogenic and environmental pressures. In this study, we developed a framework for the dynamic assessment of soil vulnerability to antibiotic contamination in urban green spaces, combining antibiotic release, exposure, and consequence layers. According to the results, soil vulnerability risks shown obvious spatial-temporal variation in urban areas. Areas at a high risk of antibiotic contamination were usually found in urban centers with high population densities and in seasons with low temperature and vegetation coverage. Quinolones (e.g., ofloxacin and norfloxacin) were priority antibiotics that posed the highest vulnerability risks, followed by tetracyclines. We also confirmed the effectiveness of the vulnerability assessment by correlating soil vulnerability indexes and antibiotic residues in urban soils. Furthermore, urbanization- and land use-related parameters were shown to be critical in regulating soil vulnerability to antibiotic contamination based on sensitivity analysis. These findings have important implications for the prediction and mitigation of urban soil contamination with antibiotics and strategies to improve human health.

A landscape source-sink model to understanding the seasonal dynamics of antibiotics in soils at watershed scale.

Human and veterinary antibiotics occur widely in soil ecosystems and pose a serious threat to soil health. Landscape structure can be linked to Earth surface processes and anthropogenic footprints and may influence the variability of antibiotics in soil. In this study, an improved landscape source-sink model was used to characterize source-sink structures using the location-weighted landscape index (LWLI), which can be linked to antibiotic seasonality. The topographic wetness index was employed to identify source and sink landscapes, which represent antibiotic transport pathways via topography-driven hydrological processes. The results indicate that LWLI values and antibiotic seasonality are typically higher in farmland soils than in forest and orchard soils. LWLI values exhibit significant positive correlations with antibiotic seasonality in soils (R2: 0.33-0.58). Furthermore, landscape source-sink structures have a significant influence on antibiotic seasonality between winter and other seasons in farmland soils; however, these structures affect antibiotic seasonality between summer and other seasons in forest and orchard soils. The results of this study indicate that water movement regulated by landscape structure may play a crucial role in influencing antibiotic seasonality in soils at the watershed scale, and the landscape source-sink model can be used to quantitatively evaluate antibiotic seasonality in soil environment.

Metabolic signatures of tear extracellular vesicles caused by herpes simplex keratitis.

PURPOSE: Herpes simplex keratitis (HSK), caused by type 1 herpes simplex virus (HSV) reactivation, is a severe infectious disease that leads to vision loss. HSV can trigger metabolic reprogramming in the host cell and change the extracellular vesicles (EV) cargos; however, little is known about the EV metabolic signatures during ocular HSV infection. Here, we aimed to depict the EV-associated metabolic landscape in HSK patients' tears. METHODS: We collected 82 samples from 41 participants with unilateral HSK (contralateral unaffected tears were set as negative control), including subtype cohorts of 13 epithelial, 20 stromal, and 8 endothelial HSK. We isolated tear EVs via our previously established platform and conducted metabolic analysis using LC-MS/MS. The metabolic signatures for recognizing HSK and subtypes were assessed through differential analysis and machine learning algorithms. RESULTS: Hypopsia and increased extracellular CD63 levels were observed in affected eyes. We identified 339 metabolites based on sEVs isolated from tears. Differential analysis revealed alterations in energy and amino acid metabolism, as well as the infectious microenvironment. Furthermore, we observed dysregulated metabolite such as methyldopa, which is associated with inappropriate neovascularization and corneal sensation loss, contributing to the HSK severity particularly in the stromal subtype. Moreover, machine learning classification also suggested a set of EV metabolic signatures that have potential for pan-keratitis detection. CONCLUSIONS: Our findings demonstrate that tear EV metabolites can serve as valuable indicators for comprehending the underlying pathological mechanisms. This knowledge is expected to facilitate the development of liquid biopsy means and therapeutic target discovery.

Reducing risks of antibiotics to crop production requires land system intensification within thresholds.

Land system intensification has substantially enhanced crop production; however, it has also created soil antibiotic pollution, undermining crop production. Here, we projected soil antibiotic pollution risks to crop production at multiple geographical scales in China and linked them to land system intensification (including arable land expansion and input increase). Our projections suggest that crop production will substantially decrease when the soil antibiotic pollution risk quotient exceeds 8.30-9.98. Land systems explain most of the variability in antibiotic pollution risks (21-66%) across spatial scales. The convex nonlinearities in tradeoffs between antibiotic pollution risk and crop production indicate that vegetable and wheat production have higher thresholds of land system intensification at which the risk-yield tradeoffs will peak than do maize and rice production. Our study suggests that land system intensification below the minimum thresholds at multiple scales is required for acceptable antibiotic pollution risks related to crop yield reduction.

An innovative risk evaluation method on soil pathogens in urban-rural ecosystem.

The presence and reproduction of pathogens in soil environment have significant negative impacts on soil security and human health in urban-rural ecosystem. Rapid urbanization has dramatically changed the land use, soil ecosystems, and the presence of pathogens in soil environment, however, the risk associated with soil pathogens remains unknown. Identifying the potential risk of pathogens in soils in urban-rural ecosystem has become an urgent issue. In this study, we established a risk evaluation method for soil pathogens based on analytic hierarchy process and entropy methods to quantitatively estimate the potential risk of soil pathogens to children and adults in urban-rural ecosystem. The abundance and species number of soil pathogens, network structure of soil microbial community, and human exposure factors were considered with 12 indicators to establish the risk evaluation system. The results revealed that 19 potential pathogenic bacteria were detected in soils within a typical urban-rural ecosystem. Substantial differences were observed in both abundance and species of soil pathogens as well as network structure of soil microbial community from urban to rural areas. Urban areas exhibited relatively lower levels of soil pathogenic abundance, but the microbial network was considerably unstable. Rural areas supported relatively higher levels of soil pathogenic abundance and stable microbial networks. Notably, peri-urban areas showed relatively unstable microbial networks alongside higher levels of soil pathogenic abundance compared to other areas. The risk evaluation of soil pathogens for both adults and children showed that peri-urban areas presented the highest potential risk, with children being more susceptible than adults to threats posed by soil pathogens in both urban and peri-urban areas. The established evaluation system provides an innovative approach for quantifying risk of soil pathogens at regional scale and can be used as a reference for preventing soil pathogens contamination and enhancing soil health in areas with intense human activities.

Long-term variations in external phosphorus inputs and riverine phosphorus export in a typical arid and semiarid irrigation watershed.

Excessive phosphorus (P) along with drained water from farmland in the arid and semiarid watersheds when entering into water bodies brings about serious environmental problems in the aquatic ecosystem. It is critical to explore variations in watershed P balance and the relationship between anthropogenic P input and riverine total phosphorus (TP) export in a typical irrigation watersheds. In this study, long-term anthropogenic P variations in Ulansuhai Nur watershed (UNW), a typical irrigation watershed in Yellow River basin, was investigated using a quantitative Net Anthropogenic Phosphorus Input (NAPI) budget model. The results showed that annual NAPI exhibited a significant upward trend with a multi-year average of 2541.6 kg P km-2 yr-1 in the UNW. Hotspots for watershed NAPI were discovered in Linhe and Hangjin Houqi counties. Chemical P fertilizers and livestock breeding were two dominated sources of NAPI. Annual riverine TP export showed a significantly declined trend with a net decrease of 80.6%. The export ratio of watershed NAPI was 0.6%, lower than those reported for other watersheds worldwide. There was a significant positive linear correlation between NAPI and riverine TP export from 2005 to 2009. However, after 2009, riverine TP export exhibited a decreased trend with increasing watershed NAPI, which was attributed to environmental treatment measures. By reconstructing riverine TP export without the impact of pollution treatment measures, annual average reduction amount of riverine TP export from 2009 to 2019 was estimated to be 237.2 ton, 47.2% and 52.8% of which were attributed to the point and nonpoint sources measures. This study not only widens the application scope of NAPI budget method, but also provides useful information of nutrient management and control in the arid and semiarid irrigation watershed.

Occurrence, spatial distribution and ecological risks of antibiotics in soil in urban agglomeration.

Antibiotics in soil environment are regarded as emerging pollutants and have introduced increasing risks to soil ecosystem and human health in rapid urbanization areas. Identifying the occurrence and spatial variability of antibiotics in soils is an urgent issue in sustaining soil security. In this study, antibiotics in soils were investigated and analyzed in Beijing-Tianjin-Hebei urban agglomeration. The occurrence, spatial distribution, and related affecting factors of antibiotics in soils were identified and ecological risks of antibiotics in soil environment were assessed. Results showed that (1) The mean concentration of soil antibiotics in Beijing-Tianjin-Hebei urban agglomeration was 21.79 µg/kg. Land use substantially affected the occurrence and concentration of antibiotics in soils. Concentrations of antibiotics in cropland and orchard soils were 2-3 times higher than the other land use types. (2) The concentrations of antibiotics in soils in Beijing-Tianjin-Hebei urban agglomeration presented a spatial pattern of high values in southeast, and low values in northwest. Spatial variability of antibiotics in soils was closely related to the application of organic fertilizer and wastewater irrigation as well as topographical features. Furthermore, soil properties and land management policy had substantial influences on soil antibiotics, and soil heavy metals may aggravate the accumulation of antibiotics in soils. (3) Ecological risks assessment of antibiotics in soils demonstrated that erythromycin (ERY), sulfamethoxazole (SMX), and doxycycline (DOX) may introduce high risks to soil ecosystem health, and more attention should be paid to the areas with intensive human activities that had potential high risk to soil ecosystem health. This study suggests that scientific land and soil management should be considered to prevent soil antibiotic pollution and sustain soil security in urban agglomeration.

An approach to quantify the dependence of economy on resource efficiency: A case study in Beijing-Tianjin-Hebei region of north China.

The improvements of resource efficiency are crucial for economic development of a city. Currently, the variations of utilization efficiency of ecological resources have been studied much, but whether the efficiency has been good enough for driving the economy, is still unknown. Here, we introduced an efficiency dependence index (EDI) to assess the effects of utilization efficiency of land, energy and water resources on economy and evaluated the promotion potentials of resource efficiency. The Beijing-Tianjin-Hebei (BTH) region of north China was selected to validate the method. First, a non-collinear index system was built. After that, the EDI and the score of resource efficiency (SRE) index were constructed based on the generalized linear regression method. The main results were: (1) The efficiency contributions to economy in primary industry (4 of 13 cities) were far weaker than those in secondary industry (10 of 13 cities). (2) Water efficiency of primary industry in developed cities need further promotions. Energy efficiency needs more improvements especially in medium and small cities in both primary and secondary industries. (3) The efficiency utilization patterns of effective efficiency (EE), potential efficiency (PE) and no efficiency (NE) were categorized, and there were 26 of 65 (40%) counts of indicators performed EE, 31% counts of indicators presented PE and 29% counts of indicators showed NE. The improvement of water efficiency was the key driver to promote the resource efficiency. (4) By comparing current and ideal resource efficiency levels (via SRE), it showed that only 1 city reached to 80% of the ideal efficiency, 3 cities showed 70%, 4 cities showed 60% and 5 cities reached to less than 50% of the ideal resource efficiency. Policy suggestions of optimizing energy structure and pay attention to the comprehensiveness of resource efficiency were provided. This paper could serve as a reference for resource management.

[Biomechanical study of polymethyl methacrylate bone cement and allogeneic bone for strengthening sheep vertebrae].

OBJECTIVE: To investigate the feasibility and mechanical properties of polymethyl methacrylate (PMMA) bone cement and allogeneic bone mixture to strengthen sheep vertebrae with osteoporotic compression fracture. METHODS: A total of 75 lumbar vertebrae (L 1-L 5) of adult goats was harvested to prepare the osteoporotic vertebral body model by decalcification. The volume of vertebral body and the weight and bone density before and after decalcification were measured. And the failure strength, failure displacement, and stiffness were tested by using a mechanical tester. Then the vertebral compression fracture models were prepared and divided into 3 groups ( n=25). The vertebral bodies were injected with allogeneic bone in group A, PMMA bone cement in group B, and mixture of allogeneic bone and PMMA bone cement in a ratio of 1∶1 in group C. After CT observation of the implant distribution in the vertebral body, the failure strength, failure displacement, and stiffness of the vertebral body were measured again. RESULTS: There was no significant difference in weight, bone density, and volume of vertebral bodies before decalcification between groups ( P>0.05). After decalcification, there was no significant difference in bone density, decreasing rate, and weight between groups ( P>0.05). There were significant differences in vertebral body weight and bone mineral density between pre- and post-decalcification in 3 groups ( P<0.05). CT showed that the implants in each group were evenly distributed in the vertebral body with no leakage. Before fracture, the differences in vertebral body failure strength, failure displacement, and stiffness between groups were not significant ( P>0.05). After augmentation, the failure displacement of group A was significantly greater than that of groups B and C, and the failure strength and stiffness were less than those of groups B and C, the failure displacement of group C was greater than that of group B, and the failure strength and stiffness were less than those of group B, the differences between groups were significant ( P<0.05). Except for the failure strength of group A ( P>0.05), the differences in the failure strength, failure displacement, and stiffness before fracture and after augmentation in the other groups were significant ( P<0.05). CONCLUSION: The mixture of allogeneic bone and PMMA bone cement in a ratio of 1∶1 can improve the strength of the vertebral body of sheep osteoporotic compression fractures and restore the initial stiffness of the vertebral body. It has good mechanical properties and can be used as one of the filling materials in percutaneous vertebroplasty.

A bone substitute composed of polymethyl-methacrylate bone cement and Bio-Gene allogeneic bone promotes osteoblast viability, adhesion and differentiation.

BACKGROUND: Increasing reports on new cement formulations that address the shortcomings of PMMA bone cements and various active components have been introduced to improve the biological activity of PMMA cement. OBJECTIVE: Evaluating the biological properties of PMMA cements reinforced with Bio-Gene allogeneic bone. METHODS: The MC3T3-E1 mouse osteoblast-like cells were utilized to determine the effects of Bio-Gene + PMMA on osteoblast viability, adhesion and differentiation. RESULTS: The combination of allogeneic bone and PMMA increased the number of adherent live cells compared to both control group and PMMA or Bio-Gene group. Scanning electron microscopy observed that the number of cells adhered to Bio-Gene + PMMA was larger than Bio-Gene and PMMA group. Compared with the control and PMMA or Bio-Gene group, the level of ALP and the number of calcium nodules after osteoinduction was remarkably enhanced in Bio-Gene + PMMA group. Additionally, the combination of Bio-Gene and PMMA induced the protein expression of osteocalcin, osterix and collagen I. CONCLUSION: The composition of PMMA and allogeneic bone could provide a more beneficial microenvironment for osteoblast proliferation, adhesion and differentiation. PMMA bone cement reinforced with Bio-Gene allogeneic bone may act as a novel bone substitute to improve the biological activity of PMMA cement.

[Relationship Between Source-Sink Landscape Pattern and Antibiotics in Surface Water in Peri-urban Watershed].

Quantitively identifying the effect of land use patterns on antibiotics in surface water has significance in maintaining water quality and protecting residents' health in urban and rural regions. In this study, a typical peri-urban watershed, located in the Yangtze River Delta, was selected as the study area. Based on surface water sampling, laboratory analysis, and source-sink landscape model (SSLM) analysis, the component and distribution characteristics of antibiotics in surface water in different sub-watersheds were analyzed. The effects of source and sink landscape patterns on antibiotic concentrations in surface water were identified. The results of this study showed substantial differences in types and concentrations of antibiotics in surface water in different sub-watersheds. The total concentrations of antibiotics in surface water ranged from 1.12 ng·L-1 to 53.74 ng·L-1. From upstream to downstream, the area of "source" landscape increased, and the area of "sink" landscape decreased based on landscape pattern analysis. The results of non-metric multidimensional scaling (NMDS) showed that sub-watersheds with similar "source-sink" landscape patterns were detected as having similar antibiotics types and concentrations in surface water. Land use composition, distance, elevation, and slope degree had substantial impacts on antibiotic concentrations in surface water. The results of this study also found that location-weighted landscape index (LWLI) was positive correlated with antibiotics concentrations in surface water based on correlation analysis and redundancy analysis. The sub-watersheds with high LWLI values usually had relatively higher antibiotic concentrations in surface water. This study indicated that optimization of "source" and "sink" landscapes at the watershed scale can decrease antibiotic contamination in surface water. Furthermore, SSLM is an effective tool in landscape optimization at the watershed scale.

CN-China: Revised runoff curve number by using rainfall-runoff events data in China.

The curve number (CN) method developed by the United States Department of Agriculture (USDA) in 1954 is the most common adopted method to estimate surface runoff. For years, applicability of the CN method is a conundrum when implementing to other countries. Specifically, countries with more complex natural environment may require more dedicated adjustments. Therefore, the current CN look-up table provided by USDA might not be appropriate and could be questionable to be applied directly to regions elsewhere. Some studies have been conducted to modify CN values according to specified natural characteristics in scattered regions of mainland China. However, an integral and representative work is still not available to address potential concerns in general matters. In this study, a large set of rainfall-runoff monitoring data were collected to adjust CN values in 55 study sites across China. The results showed that the revised CN values are largely different from CN look-up table provided by USDA, which would lead to huge errors in runoff estimation. In this study, the revised CN (dubbed CN-China) provides better reference guidelines that are suitable for most natural conditions in China. In addition, scientists and engineers from other parts of the world can take advantage of the proposed work to enhance the quality of future programs related to surface runoff estimation.

Multimedia mass balance approach to characterizing the transport potential of antibiotics in soil-plant systems following manure application.

Antibiotics are ubiquitous in agro-ecosystems worldwide, which can pose remarkable risks to ecological security and human health. However, comprehensive evaluation on the multimedia fate and transport potential of antibiotics in soil-plant systems is still lacking. A mass balance approach was performed to gain insights into the transport and fate of antibiotics in soil-plant systems following manure application. Our results showed that more than 99 % of antibiotics were released from applied manure fertilizer into the soil-plant system. Antibiotic concentrations in soil and plant compartments increased over 120 days. Most of the antibiotics persisted in soil (about 65 %), while less than 0.1 % accumulated in the plants. Rainfall-induced runoff, subsurface interflow and soil water infiltration were alternative transport pathways for antibiotics in soil-plant systems although their contributions were limited. Dissipation was the main removal pathway for antibiotics accounting for about 33 % of total input mass. Tetracyclines had higher mass proportion in soil following by quinolones, whereas most of sulfonamides and macrolides were dissipated. Mass balance approach based on tracking environmental fates of antibiotics can facilitate the understandings in the source comparisons and mitigation strategies, and therefore provide insights to inform modeling and limiting the transport of manure-borne antibiotics to neighboring environmental compartments.

Effects of land use and rainfall on sequestration of veterinary antibiotics in soils at the hillslope scale.

Veterinary antibiotics have been detected as contaminants of emerging concern in soil environment worldwide. Animal manure is frequently applied to agricultural fields to improve soil fertility, which can result in introducing large amount of antibiotics into soil environment. However, few attempts have been made to identify the spatial and temporal dynamics of veterinary antibiotics in soil at the hillslope scale with different land uses. This study was performed to explore the pattern and variability of veterinary antibiotics in the soil in response to rainfall events. Results showed that higher concentrations of veterinary antibiotics were generally found in cropland (292.6 ± 280.1 ng/g) and orchard (228.1 ± 230.5 ng/g) than in forestland (13.5 ± 9.9 ng/g). After rainfall events, antibiotics accumulated in the soil at the positions where manure was applied, especially under high-intensity rainfall conditions. However, the antibiotic concentration in soil slightly increased from the top to the bottom of hills, thus indicating the restricted contribution of runoff to antibiotic transport, especially under low-intensity rainfall conditions. In addition, most antibiotics were sequestered in the surface soil (0-10 cm), and higher antibiotic concentrations were observed in deep soil (20-40 cm) in cropland than orchard. The soil aggregate, organic matter, and clay content played important roles in antibiotic sequestration along the hillslope subject to low-, medium-, and large-amount rainfall events, respectively. This study identified that land use, rainfall conditions, and soil structures jointly affect the spatial and temporal variability of antibiotics in soils on hillslopes.

Simulation of the spatial stresses due to territorial land development on Yellow River Delta Nature Reserve using a GIS-based assessment model.

This study aimed at assessing the stresses from land development in or around Yellow River Delta Nature Reserve (YRDNR) and identifying the impacted areas. Major land development types (reservoirs, pond, aquafarm, salt pan, road, residential land, industry land, farming land, and fishing land) in or around the YRDNR from 1995 to 2014 were identified using spatial data sets derived from remote sensing imageries. The spatial stresses were simulated by considering disturbance due to land development activities and accessibility of disturbance using a geographic information system based model. The stresses were then used to identify the impacted area by land development (IALD). The results indicated that main increasing land development types in the study area from 1995 to 2014 were salt pan and construction land. The 98.2% of expanded land development area and 93.7% of increased pump number showed a good control of reserve function zone on land development spread. The spatial stress values and percentages of IALD increased from 1995 to 2014, and IALD percentage exceeded 50% for both parts of YRDNR in 2014. The results of this study also provided the information that detailed planning of the YRDNR (2014-2020) could decrease the spatial stress and IALD percentage of the whole YRDNR on the condition that the area of land development activities increased by 24.4 km2 from 2014 to 2020. Effective measures should be taken to protect such areas from being further disturbed in order to achieve the goal of a more effective conservation of the YRDNR, and attention should be paid to the disordered land development activities in or around the natural reserves.